In donkey epididymis tissue, miR-143-3p, miR-711, miR-200b-3p, miR-761 and miR-429-3p regulated 18 genes (Figure 6C, Supplementary Table S5), and the genes were enriched in the pathway of “TGF-β signaling pathway”, “mucin type o-glycan biosynthesis” and “melanoma”, which may be regulated by miR-143-3p and miR-711 through BMP5, FGF7, GCNT1 and THSD4 (Figure 6D). The gene discussed is TGFB1; the disease is melanoma.