Several metabolic regulatory pathways participated in cancer, such as the proteoglycans in cancer pathway (ecb05205, e.g., TWIST2, HGF, EGFR), choline metabolism in cancer pathway (ecb05231, e.g., PLA2G4A, PLD1) and central carbon metabolism in cancer pathway (ecb05230, e.g., LDHB, HK2), were also enriched. The gene discussed is TWIST2; the disease is cancer.