Regarding the role of these lncRNAs in the development and progression of lung cancer, and due to a lack of experimental evidence about the effect of these lncRNAs as cargos of extracellular vesicles, the present study aimed to evaluate the differential expression of 5 lncRNAs including GHSROS, HMlincRNA717, HNF1A-AS1, HOTAIR, and LINCRNA-p21 in the tumors, tumor-derived exosomes, and ANCT in NSCLC patients. The gene discussed is HOTAIR; the disease is lung cancer.