NUP98 and acute myeloid leukemia: Therefore, the present study was designed with the following objectives: (i) to develop a RT-qPCR-based assay to detect and determine the copy number of NUP98::NSD1 [t(5;11)(q35;p15.5)] transcript with high sensitivity and specificity; (ii) to use this assay for routine screening to find out the presence of this fusion transcript in AML patients.