We then evaluated the relationship of these 16 enzymes with tumor invasion and the prognosis of colorectal cancer, and we determined that there were eight amino acid enzymes that have a key effect on the prognosis and staging of colorectal cancer.: ACADM, ACADS, GPX4, GSR, HADH, HMGCL, HMGCS1 and IDH1. Here, HMGCL is linked to colorectal cancer.