PRF1 and neoplasm: To assess whether the increased MC38 tumor burden and alternatively activated endotype of TAMs observed in LysMCre/+;Mycfl/fl hosts could be attributed to reduced activity of CD8+ effector T-cells, we next assessed the expression of cytotoxic molecules (i.e., GzmB and Prf1) in CD8+ T-cells isolated from MC38 tumors of LysMCre/+;Mycfl/fl and LysM+/+;Mycfl/fl hosts (Figure 4D).