Based on the qPCR and Western blot analyses, we selected MCF-7, a simple epithelial breast carcinoma cell line devoid of endogenous vimentin to recapitulate the EMT process and ectopically expressed the full-length vimentin (pLPChygro-FV) or the control vector (pLPChygro-CV). This evidence concerns the gene VIM and breast carcinoma.