TSC1 and Down syndrome: In other studies, hESCs-derived neurons have been used: to model FOXG1 syndrome to control the endogenous protein dosage of FOXG1 protein in a precise manner which is important for GABA interneuron differentiation [37]; to assess the effect of high glucose concentration in masking the TSC cellular phenotypes using a hESCs-derived TSC model [38]; to investigate genotoxicity of anti-seizure medications [39], and the mode of action of the ASM valproate [40].