In this respect, the transfection of Cx43 into human CRC HT29 cells, which do not express the Cx43 protein, reduced the growth of these cells in monolayer and tumor xenografts by partly colocalizing with β-catenin and downregulating the Wnt signaling pathway, which is frequently activated in colon carcinogenesis [35]. This evidence concerns the gene GJA1 and colorectal carcinoma.