We focused on VCAM-1, iNOS, and COX-2 expression since we knew they played a role in periodontal disease development [12,13,14]. Figure 3 demonstrates that iNOS expression was inhibited by 3.75–15 μM iberin, VCAM-1 expression also suppressed 7.5 and 15 μM iberin treatment, and 7.5 μM iberin clearly down-regulated COX-2 expression in TNF-α-stimulated TR146 cells. Here, NOS2 is linked to periodontal disorder.