Acridine orange/propidium iodide cell viability assays showed no loss of cell viability with APX3330 (50 or 100 μM), APX2009 (5 μM), or APX2014 (5 μM) in both Tsc2−/− MEFs and 621-102 AML cells, while some loss of cell viability became apparent using the higher APX2014 concentration of 10 μM (data not shown). The gene discussed is TSC2; the disease is acute myeloid leukemia.