Previously, results from our group showed that 10−7 M of ouabain modulates the expression of vimentin, TWIST and MMP9 in papillary carcinoma TPC-1 and BCPAP human thyroid cell lines [16]; however, in 8505C cells, only an increase in N-cadherin mRNA expression was observed in ouabain-treated cells after 24 h (Figure 6a–d). Here, TWIST1 is linked to thyroiditis.