Interestingly, in the cytosolic fractions, the EGFR protein levels were increased in the GBM cells transfected with the Y845F variant of EGFR/EGFRVIII compared to the control cells, but they were not revealed by the specific antibody (pEGFRY845) that recognizes phosphorylated EGFR on tyrosine 845 (Figure 4B). This evidence concerns the gene EGFR and glioblastoma.