The test contains 61 mutation sites of 16 common mutant genes, such as PIK3CA, APC, EGFR, TP53, and KRAS, and 41 cancer-specific protein biomarkers, such as AFP, CA-125, sHER2/sEGFR2/sErbB2, etc. The mutation is mainly used to analyze whether there is cancer to improve the detection sensitivity, and the tumor-specific protein biomarkers can be used for cancer types, improving the specificity of detection (>99%). The gene discussed is AFP; the disease is cancer.