Further examination of the expression of EBF3-AS in the hippocampus of AD model mice revealed that the expression of EBF3-AS was upregulated in the hippocampal tissue of APP/PS1 mice compared with C57BL/6 mice, and the mRNA and protein expression of EBF3 was upregulated together with the expression of EBF3-AS, suggesting that EBF3 may be a downstream target gene of EBF3-AS. Here, EBF3 is linked to Alzheimer disease.