This study provided additional evidence linking NAFLD to the development of CVD, since animals in the NAFLD group presented at the same time more significant liver disease, including a higher liver collagen deposition, and features of metabolic disease, with marked adipokines and insulin abnormalities, higher atherogenic ratios and paracrine disbalance, as well as a lower number of normal cardiomyocytes in comparison to controls. Here, INS is linked to metabolic dysfunction-associated steatotic liver disease.