In the present study, after the examination of B7-H3 expression profile across various cancer types including CP, we established for the first time an in vitro organoid model of CP using fresh tissue biospecimens, and based on the model, we evaluated the antitumor efficacy of B7-H3-targeted CAR-T cells and ADC against craniopharyngioma. The gene discussed is CD276; the disease is craniopharyngioma.