LMNA and cardiomyopathy: We assessed whether SRF regulates α-tubulin acetylation by controlling ATAT1 expression in cardiomyopathy caused by mutations in LMNA. To assess the impact of SRF downregulation on ATAT1 transcription, we used a luciferase reporter plasmid with a canonical MRTF-A/SRF-CArG sequence upstream of a minimal ATAT1 promoter (schematically shown in Fig. 4b).