Activationof pro-migratory molecules, such as Src and Rock1, inhibition of typeI interferon mediating anti-tumor immune response, and induction ofHCC markers, such as Afp and Gpc3, indicate that AEG-1-C75S mice mayharbor a pro-tumorigenic milieu, and we speculate that with age, thesemice might develop spontaneous tumors or if treated with a carcinogen,such as DEN, they might develop aggressive, metastatic tumors comparedto their AEG-1-WT counterparts. This evidence concerns the gene ROCK1 and metastatic neoplasm.