In order to further explore the molecular mechanism whereby MC-LR aggravates liver lipid metabolism disorders in obese mice fed a high-fat diet, the WB method was used to detect the protein expression levels of phosphoinositide 3-kinase (PI3K), phosphorylation phosphatidylinositol 3-kinase (p-PI3K), protein kinase B (AKT), phosphorylation protein kinase B (p-AKT), mammalian target of rapamycin (mTOR), phosphorylation mammalian target of rapamycin (p-mTOR), and sterol regulatory element binding protein 1c (SREBP1-c). This evidence concerns the gene SREBF1 and Disorder of lipid metabolism.