The clinician could not differentiate early, active, latent, chronic, congenital, or reactivated infection from serological assays, such as latex agglutination assay (LAT), immunochromatographic techniques (ICT), and enzyme linked immunosorbant assay (ELISA) (Table 7), which are used for qualitative and quantitative detection of IgM and IgG antibodies, but cannot differentiate between acute and chronic persistent infection of T. gondii parasite. Here, CD40LG is linked to infection.