The expression levels of the markers, including c-kit, nNOS, NSE, and PGP9.5 proteins, were measured in the mid colon of the Lep KO and HFD-treated mice to determine if the loss of ICC, nitrergic enteric, and myenteric neurons were similar in both models. The gene discussed is UCHL1; the disease is intrahepatic cholangiocarcinoma.