Since mTOR signal is a well-known negative regulator of autophagy (51), we then determined the effect of the UBE2C/CDH1/DEPTOR axis on autophagy and found that knockdown of either UBE2C or CDH1 induced autophagy, as demonstrated by enhanced LC3 immunofluorescent staining, LC3-I to LC3-II conversion, and p62 degradation in both H358 and H1975 lung cancer cells, which were partially rescued by simultaneous knockdown of DEPTOR (Figure 6E and Supplemental Figure 6, E–G). Here, MAP1LC3A is linked to lung cancer.