Since DEPTOR is known to inhibit both mTORC1 and mTORC2 (33), we measured DEPTOR levels and found that they were indeed increased upon UBE2C knockdown in both cell lines (Figure 3A), as well as in 2 additional lines of lung cancer cells (Supplemental Figure 3B) and MEFs (Supplemental Figure 3C). Here, DEPTOR is linked to lung carcinoma.