To identify the signaling pathways of the seven immune-related genes in CVD and COVID-19, GSEA between high-and low-C5AR1, MMP9, CYBB, FPR2, CSF1R, TLR2, and TLR4 expression matrices was performed to recognize signaling pathways based on h.all.v7.4.entrez.gmt, collected in MSigDB. The gene discussed is TLR4; the disease is COVID-19.