FN1 and familial pancreatic carcinoma: To understand how pancreatic cancer cells respond to substrate stiffness, we cultured two independent PDAC cell lines, PDAC-A and PDAC-B, from KPC mice (Pdx1-Cre; LSL-KrasG12D/+; LSL-Trp53R172H/+; Morton et al., 2010), on fibronectin-coated polyacrylamide hydrogels of three defined stiffnesses, as reported previously (Papalazarou et al., 2020), and investigated gene expression profiles by performing RNA sequencing (Fig. 1A).