Up‐regulation of Tim‐3 in LSCs at the level of gene and protein expression is associated with poor prognosis and the important biological feature of Tim‐3 of LSC in AML.;Up regulated genes in Tim‐3high group were mainly enriched in immune response, cytokine binding and cell adhesion molecules, and JAK‐STAT signaling pathway, by gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Here, HAVCR2 is linked to acute myeloid leukemia.