Interestingly, when we look into the relationship between the genes and top upregulated pathways, we identified TLR genes such as TLR1, TLR3, TLR6 and TLR9 as connector genes amongst different pathways both in SY18 and HuB20 infection (Figure 4B), highlighting the upregulation of TLRs as key genes in the host response to ASFV infection. The gene discussed is TLR3; the disease is infection.