To address a potential impact of CAF and PtF populations on the functionality of cancer and endothelial cells, cytokine secretion of vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor BB (PDGF-BB), transforming growth factor β2 (TGF-β2), and brain-derived neurotrophic factor (BDNF) by CAFs and PtFs was measured by ELISA. The gene discussed is BDNF; the disease is cancer.