Representative images of ICC-stained samples show that CAFs, PtFs, and hOFs have a mesenchymal, spindle-shaped cell morphology and lack the expression of epithelial markers E-cadherin and pan-cytokeratin, and the endothelial marker CD31 (Figure 2A, Supplementary Figure 1). The gene discussed is CDH1; the disease is intrahepatic cholangiocarcinoma.