To further investigate the response to PD-1 blockade, we detected the expression level of T cell activation associated marker: CD69.19,20 In ccRCC-07_PDO, the expression level of CD69 in CD8+ T cells was significantly increased under toripalimab treatment, which was consistent with the increased ratio of CD8+ T cells, indicating the rescue of T cell exhaustion state in the presence of PD-1 blockade. Here, CD69 is linked to nonpapillary renal cell carcinoma.