Figure 4 shows the expression of the transcription factors RORγT, Foxp3, PPARγ, and NF-κB. RORγT expression was not significantly different between the infected CLA and control groups, but there was a significant difference for the PBS group at 6 dpi compared with 0 dpi (P=0.016) (Figure 4). Foxp3 expression was increased at 6 dpi in both groups (P<0.05). At 40 dpi in the CLA group, Foxp3 was higher compared with 0 dpi (P=0.001) (Figure 4). CLA supplementation or infection evolution had no effect on PPARγ or NF-κB (Figure 4). This evidence concerns the gene PPARG and infection.