Third, although our results were generated from similar sample size to previous publications using Visium platform [16, 74, 75], further SRT analyses of larger sample size without biological covariates (e.g. age, post-mortem interval, RNA integrity, tissue intactness and ApoE genotype), sex differences and additional functional studies are warranted for fully understanding the relationship between transcriptional changes and various AD pathology. The gene discussed is APOE; the disease is Alzheimer disease.