Furthermore, we validated a few novel DEGs identified in this study, including KIF5A, PAQR6, and SLC1A3. We found that the RNA level of KIF5A was significantly increased in microglia, astrocytes, and neurons in layers II/III and V and layer V oligodendrocytes of the MTG in human AD compared to CT cases. The gene discussed is KIF5A; the disease is Alzheimer disease.