EBLN3P and osteosarcoma: We selected 2 osteosarcoma cell lines, MNNG/HOS and MG63 with relatively lower EBLN3P expressions used for further MTX-resistant cell establishment, and finally, the MTX-resistant cell line was successfully established, and IC50 increased from 0.2 to 1 μM for MNNG/HOS, and 0.35 to 1.3 μM for MG63 (Fig. 1F, G).