Four osteosarcoma cell lines were used in the current study, and their IC50 of MTX were screened by MTT assay, which is shown in Fig. 1C. Meanwhile, the expression of LncRNA EBLN3P in each cell lines was also examined by qRT-PCR, which is shown in Fig. 1D. As shown in Fig. 1E, the MTX IC50 for each cell lines were positively correlated with RNA expression of EBLN3P, also suggest that higher expression of EBLN3P might be associated with MTX resistance. This evidence concerns the gene EBLN3P and osteosarcoma.