More strikingly, we showed that adoptive transfer of macrophages that were pre-conditioned with HMGB1-depleted LCM yielded much smaller tumors than those pre-treated with HMGB1-sufficient LCM (Fig. S8a, b), highlighting the significance of tumor-derived HMGB1 in promoting the generation of pro-tumor macrophages. Here, HMGB1 is linked to neoplasm.