The results of the present study showed that the expression levels of mRNA and protein of HIF-1α were higher in all groups of hypoxia-ischemia than in the control group, and were higher in the group with severe hypoxia-ischemia (30 min) than in the group with mild Hypoxia (10 min), with the highest at 30 min of reperfusion. This evidence concerns the gene HIF1A and ischemia.