Therefore, we analyzed public ChIP-seq data (GSE94822) and found that MYCN, GATA3, PHOX2B and HAND2 (TFs in CRC) shared peaks with H3K27ac peaks at the enhancer region of SMAD9 in MYCN-amplified BE(2)-C cells (Fig. 2D). This evidence concerns the gene HAND2 and colorectal carcinoma.