GJA1 and cancer: Furthermore, functional enrichment analysis performed in IPA of predicted target mRNAs by TargetScan36 for the top 20 enriched pathways for all dysregulated miRNAs in Cx43-KO-S1 cells compared to S1 cells revealed that Protein Kinase A signaling, ERK/MAPK signaling, signaling by Rho GTPases, inflammation by IL-8 signaling and regulation of epithelial to mesenchymal transition (EMT), hence well-known molecular mechanisms of cancer, were among the most enriched pathways in the differentially expressed miRNAs upon Cx43 loss (Fig. 1c).