miR-183-5p was chosen as it was up-regulated in Cx43-KO-S1 cells, was the most up-regulated miRNA in the early-stage Lebanese breast cancer patient cohort, was up-regulated in matched patients from TCGA10, and its up-regulation conferred with the increased risk of cancer progression in the 3D culture model. This evidence concerns the gene GJA1 and breast carcinoma.