The most upregulated common miRNA in breast cancer patient samples (fold change = 10) and Cx43-KO-S1 cells (fold change = 1.79) was miR-183-5p, followed by miR-182-5p (fold change = 5.96 in patients and 1.62 in cells), followed by miR-663a (fold change = 1.56 in patients and 1.46 in cells) (Fig. 1d),16. Here, GJA1 is linked to breast cancer.