The results confirmed that JAG1 was significantly associated with lymph node metastasis (χ2 = 15.034, P < 0.001), distant metastasis (χ2 = 11.148, P = 0.001), and the TNM stage (χ2 = 12.215, P = 0.002) (Table 1). This evidence concerns the gene JAG1 and metastatic malignant neoplasm in the lymph nodes.