Next, immunocompetent and immunodeficient mice were injected separately and subcutaneously with pancreatic cancer cells pretreated with DMSO or a USP8-specific inhibitor (1 μM, 24 h), to further assess the impact of inhibiting USP8 on pancreatic cancer tumorigenesis and development (Fig. 2k). This evidence concerns the gene USP8 and familial pancreatic carcinoma.