Therefore, to measure the contribution of these two pathways to the generation of glutamate and aspartate in ccRCC cells in Plasmax, we performed a 13C5 glutamine tracing experiment in the presence of CB-839, a glutaminase inhibitor39 and BMS-303141, a selective inhibitor of ATP Citrate Lyase40 (ACLY, Supplementary Fig. 6a). Here, GLS is linked to nonpapillary renal cell carcinoma.