SPRED1 and neoplasm: In the cancer setting, such non-germline, mosaic F0 approaches have been very important in identifying oncogenes (i.e. SETDB1 and CRKL) (Ceol et al., 2011; Weiss et al., 2022) and tumor suppressors (i.e. SPRED1) (Ablain et al., 2018) and can be used to test effects in thousands of animals per experiment, a major benefit of the zebrafish system.