Interestingly, although the absence of IGFL2‐AS1 clearly impairs HT29 and LoVo cell viability when the overexpressing CA9 vector was co‐transfected with shIGFL2‐AS1#1, the inhibition of CRC cell viability by IGFL2‐AS1 knockdown was blocked as CA9 level increased (Figure 5A). This evidence concerns the gene CA9 and colorectal carcinoma.