To further clarify the influence of Aβ plaques and tau tangles in microglia pathophysiology during late-stage AD, we unbiasedly subdivided subsetted microglia populations into five distinguishable clusters across all four genotypes (WT, APP;PS1, Tau4RΔK, and Tau4RΔK-AP) in 12-month-old mice. Here, APP is linked to Alzheimer disease.