We have identified a robust Lgals3 expression in LADAM in 12-month-old Tau4RΔK mice (Fig. 2), and Lgals3 levels were associated with the severity of tau aggregation (Figure S11), suggesting that Lgals3 could also be induced by Tauopathy in a sub-population of LADAM that reside near the corpus callosum.In addition, we have validated that EADAM marker, STAT1, is robustly expressed in microglia in Tau4RΔK-AP mice at 6-month-old and 12-month-old (Figure S12), but not expressed in astrocytes, which is consistent with our scRNA-Seq finding (Fig. 1). Here, MAPT is linked to tauopathy.