To determine whether the alteration of LSD1 level is associated with the transcriptional repression in neurons, we overexpressed LSD1 transiently and measured Gal4-DBD-driven luciferase activity as shown in Fig. 1G. This result shows LSD1 expression suppresses transcription in transiently transfected SH-SY5Y neuroblastoma cells suggesting that LSD1 expression can regulate transcriptional activity in neurons. This evidence concerns the gene KDM1A and neuroblastoma.