The difference in PE in the Kupffer phase between HCC lesions and distal liver parenchyma < − 40,000,000 a.u. may contribute to a more accurate diagnosis of the high Ki-67 expression group, and the sensitivity and specificity for distinguishing the high Ki-67 expression from the low Ki-67 expression group was 82.9% and 38.7%, respectively (Tables 3) (Fig. 5). This evidence concerns the gene MKI67 and hepatocellular carcinoma.