In the RT-QuIC reaction, small amounts of a misfolded PrPSc seed which can be derived from the brain, cerebrospinal fluid (CSF), olfactory mucosa (OM) brushings, or other tissues from CJD patients (Atarashi et al. 2011; Orrú et al. 2015a, 2014) may bind and convert rec PrP substrate molecules by changing their conformation and integrating them in an amyloid aggregate. The gene discussed is PRNP; the disease is Creutzfeldt Jacob disease.