This study was the first report to perform a comprehensive comparison of the intratumoral spatial distribution between 18F-FDG and 18F-BPA PET using several non-spatial and spatial parameters for squamous cell carcinoma, melanoma, and rhabdomyosarcoma, which would be expected to either show higher uptake on 18F-BPA PET or utilize in the clinic, to verify whether 18F-FDG PET could be utilized for selection indicator for BNCT. This evidence concerns the gene DST and melanoma.