Analysis of splicing factors with conserved cross-linking peaks within and surrounding AS exons in human liver cells17 identified eight splicing factors (U2AF2, RBFOX2, QKI, hnRNPC, PCBP2, TIA1, hnRNPM and TAF15) as the top 20% ranking factors in all three comparisons analysed: feeding and fasting cycles (Fig. 1e), HFD-induced obesity (Fig. 1f) and HFr-induced obesity (Fig. 1g). Here, PCBP2 is linked to obesity due to melanocortin 4 receptor deficiency.