In order to analyze the effects of brain endothelial overexpressing Ascl1 on neurogenesis after stroke, Tie2-Cre and VE-cadherin-Cre mice were injected with AAV-FLEx-GFP or AAV-FLEx-ASCL1-GFP and then subjected to permanent occlusion of their distal middle cerebral arteries (Fig. 6d, Supplementary Fig. 8c). Here, ASCL1 is linked to Stroke.