Hepatocyte-specific deletion of Smo caused hepatic steatosis (Figure 1), insulin resistance (Figure 2), and lipotoxicity (Figure 8) in our mouse model; single-cell analysis linked these liver phenotypes with dynamic changes in hepatocyte gene expression and pathway activities that occurred in Smo-KO hepatocytes with inhibited Hedgehog signaling (Figures 6 and 7). Here, SMO is linked to Insulin resistance.