Therefore, the detection of caspase-3 is of great significance for apoptosis imaging and the evaluation of early tumor treatment and other diseases (Lei et al., 2022; Mahshid et al., 2022).RA is alleviated by inhibiting TNF-α-induced caspase3/GSDME-mediated pyroptosis (Zhai et al., 2022).Caspase-3 is a key proenzyme in apoptosis (He et al., 2014; Asadi et al., 2021), and a previous study reported that osteoclast differentiation requires activation of caspase-3 (Szymczyk et al., 2006). The gene discussed is TNF; the disease is rheumatoid arthritis.